Written by the Fiveable Content Team • Last updated August 2025
Verified for the 2027 exam
Verified for the 2027 exam•Written by the Fiveable Content Team • Last updated August 2025
Definition
A molecular technique that measures the accumulation of PCR products in real time using fluorescent signals, with the Cq value indicating the cycle at which fluorescence exceeds background, inversely related to starting mRNA amount.