In vitro assays

Definition

In vitro assays are experimental procedures conducted in a controlled environment outside of a living organism, often in petri dishes or test tubes. They are used to study biological processes, including the immune response.

5 Must Know Facts For Your Next Test

1. In vitro assays help in analyzing the function and efficacy of polyclonal and monoclonal antibodies.
2. They are essential for screening potential therapeutic agents before clinical trials.
3. Common types of in vitro assays include ELISA, flow cytometry, and Western blotting.
4. These assays provide crucial data for understanding antigen-antibody interactions.
5. In vitro conditions can be precisely controlled to isolate specific variables affecting the immune response.

Review Questions

Related terms

ELISA:

Enzyme-linked immunosorbent assay, used for detecting and quantifying substances such as proteins and antibodies.

Flow Cytometry:

A technique used to detect and measure physical and chemical characteristics of cells or particles.

Western Blotting:

A method for detecting specific proteins in a sample through gel electrophoresis followed by transfer to a membrane.