ap biology - biotechnology: pcr, crispr & cloning

timestamps

• 0:00

  Introduction to Biotechnology

• 0:32

  Biotech Overview and Applications

• 1:34

  Bacterial Transformation Lab

• 2:34

  Main Biotech Techniques

• 3:35

  CRISPR and Current Events

• 4:36

  Polymerase Chain Reaction

• 5:37

  PCR Primers and Binding

• 7:09

  PCR Cycles and Amplification

• 8:41

  PCR Purpose and Uses

• 9:42

  Restriction Enzymes Introduction

• 10:46

  Sticky Ends in DNA

• 13:52

  Restriction Enzymes After PCR

• 15:26

  Plasmids in Bacteria

• 18:32

  Recombinant DNA Creation

• 19:33

  Bacterial Transformation Process

• 21:38

  Transformation Techniques

• 24:44

  Selecting Transformed Bacteria

• 27:49

  Gel Electrophoresis Overview

• 29:54

  Gel Pores and DNA Charge

• 31:25

  DNA Size Separation

• 32:58

  Paternity Test Example

• 36:09

  Green Fluorescent Protein

• 38:41

  Genetically Modified Organisms

• 40:12

  Plasmid Definition and Use

• 41:14

  FRQ Example Analysis

• 44:06

  Inserting Gene of Interest

• 45:44

  Identifying Recombinant Bacteria

• 47:48

  Benefits and Threats Discussion

• 50:25

  Determining Transformation Success

• 51:27

  Conclusion and Preview