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  3. AP Biology - Biotechnology: PCR, CRISPR & Cloning

ap biology - biotechnology: pcr, crispr & cloning

originally streamed in 2019 with Caroline KoffkeTeacher
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Learn how biotechnology works in AP Biology with techniques like PCR, CRISPR, gel electrophoresis, and DNA cloning. This review walks you through restriction enzymes, plasmids, bacterial transformation, and real-world applications in medicine and agr ...more

curriculum note

The FRQ example used in this video is from the 2007 AP Biology exam, before the 2013 course redesign. The exam format, rubric structure, and some question expectations may differ from the current AP Biology exam.

covered in this stream

units
Unit 6 – Gene Expression and Regulation
topics
6.8 BiotechnologyBiotechnology - Slidestransformation labPresentation Slides, Transformation Lab

timestamps

  • 0:00

    Introduction to Biotechnology

  • 0:32

    Biotech Overview and Applications

  • 1:34

    Bacterial Transformation Lab

  • 2:34

    Main Biotech Techniques

  • 3:35

    CRISPR and Current Events

  • 4:36

    Polymerase Chain Reaction

  • 5:37

    PCR Primers and Binding

  • 7:09

    PCR Cycles and Amplification

  • 8:41

    PCR Purpose and Uses

  • 9:42

    Restriction Enzymes Introduction

  • 10:46

    Sticky Ends in DNA

  • 13:52

    Restriction Enzymes After PCR

  • 15:26

    Plasmids in Bacteria

  • 18:32

    Recombinant DNA Creation

  • 19:33

    Bacterial Transformation Process

  • 21:38

    Transformation Techniques

  • 24:44

    Selecting Transformed Bacteria

  • 27:49

    Gel Electrophoresis Overview

  • 29:54

    Gel Pores and DNA Charge

  • 31:25

    DNA Size Separation

  • 32:58

    Paternity Test Example

  • 36:09

    Green Fluorescent Protein

  • 38:41

    Genetically Modified Organisms

  • 40:12

    Plasmid Definition and Use

  • 41:14

    FRQ Example Analysis

  • 44:06

    Inserting Gene of Interest

  • 45:44

    Identifying Recombinant Bacteria

  • 47:48

    Benefits and Threats Discussion

  • 50:25

    Determining Transformation Success

  • 51:27

    Conclusion and Preview

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© 2026 Fiveable Inc. All rights reserved.

AP® and SAT® are trademarks registered by the College Board, which is not affiliated with, and does not endorse this website.

every AP exam is fiveable

Fiveable

Study Content & Tools

Study GuidesFRQs + AI ScoringAP Essay Grader for StudentsAI Grading for TeachersPractice QuestionsGlossaryScore Calculators

Company

What's New?PricingBulk DiscountsTestimonialsFAQsEmail us

Resources

AP Exam Schedule 2027AP Exam AccommodationsAP ClassesAP ClassroomEasiest AP ClassesHardest AP ClassesAP vs IBAP vs Dual EnrollmentAP vs Honors ClassesAP Scholar Awards

history

🌎 ap world history🇺🇸 ap us history🇪🇺 ap european history

social science

✊🏿 ap african american studies🗳️ ap comparative government🚜 ap human geography💶 ap macroeconomics🤑 ap microeconomics🧠 ap psychology👩🏾‍⚖️ ap us government

english & capstone

✍🏽 ap english language📚 ap english literature🔍 ap research💬 ap seminar

arts

🎨 ap art & design🖼️ ap art history🎵 ap music theory

science

🧬 ap biology🧪 ap chemistry♻️ ap environmental science🎡 ap physics 1🧲 ap physics 2💡 ap physics c: e&m⚙️ ap physics c: mechanics

math & computer science

🧮 ap calculus ab♾️ ap calculus bc📐 ap precalculus📊 ap statistics💻 ap computer science a⌨️ ap computer science p

world languages

🇨🇳 ap chinese🇫🇷 ap french🇩🇪 ap german🇮🇹 ap italian🇯🇵 ap japanese🏛️ ap latin🇪🇸 ap spanish language💃🏽 ap spanish literature

career kickstart

💼 ap business with personal finance🔒 ap cybersecurity

go beyond AP

high school exams

✏️ PSAT🎓 Digital SAT🎒 ACT

honors classes

🍬 honors algebra II🫀 honors anatomy🐇 honors biology👩🏽‍🔬 honors chemistry💲 honors economics📐 honors geometry⚾️ honors physics📏 honors pre-calculus📊 honors statistics🗳️ honors us government🇺🇸 honors us history🌎 honors world history

college classes

👩🏽‍🎤 arts👔 business🎤 communications🏗️ engineering📓 humanities➗ math🧑🏽‍🔬 science💶 social science

hs classes

🏛️ alabama history⛰️ appalachian studies🌴 california history🌍 earth science🌐 ethnic studies🐊 florida history🍑 georgia history🌎 global studies🌺 hawaiian studies📰 journalism🪶 native american studies🌵 new mexico history🤠 oklahoma history⚗️ physical science🎙️ speech and debate🤝 texas government🤠 texas history🌲 washington state history🕊️ world religions
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© 2026 Fiveable Inc. All rights reserved.

AP® and SAT® are trademarks registered by the College Board, which is not affiliated with, and does not endorse this website.