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  3. AP Biology - Transformation Lab: pGLO & Efficiency

ap biology - transformation lab: pglo & efficiency

originally streamed in 2021 with Jessica NadzamTeacher
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Learn how to design and analyze bacterial transformation labs for AP Biology Unit 6. This review covers transformation protocols, plasmid function, antibiotic resistance markers, and how to calculate transformation efficiency for your exam. Bacteria ...more

curriculum note

This video references the 2020 COVID AP Biology exam format (open-book, ~45 minutes total with 25 and 15-minute questions). The current AP Biology exam format is significantly different — check College Board for up-to-date exam structure and timing.

covered in this stream

units
Unit 6 – Gene Expression and Regulation
topics
6.1 DNA and RNA Structure 6.3 Transcription and RNA Processing6.4 Translation6.5 Regulation of Gene Expression6.8 BiotechnologyBacteria & Virusestransformation labGene RegulationDNA Replication, Transcription, and Translation

timestamps

  • 0:00

    Introduction and Welcome

  • 0:36

    AP Bio Exam Updates

  • 1:37

    Open Book Exam Advice

  • 3:12

    Studying Tips and Resources

  • 5:16

    Other AP Subject Streams

  • 7:20

    Practicing Application Questions

  • 8:24

    Focus on Labs for FRQs

  • 11:01

    Heredity Overview

  • 12:04

    Gregor Mendel and Genetics

  • 13:07

    DNA Structure Discovery

  • 14:08

    Genes as Recipes

  • 16:12

    Gene Expression Basics

  • 18:15

    Bill Nye Genes Video

  • 19:16

    Central Dogma Review

  • 20:18

    Transcription Process

  • 23:34

    Post-Transcriptional Processing

  • 24:36

    Prokaryotic vs Eukaryotic Expression

  • 26:41

    Alternative Splicing

  • 28:44

    Translation Overview

  • 30:16

    Gene Expression Regulation

  • 31:20

    Transcription Factors

  • 34:56

    Prokaryotic Regulation

  • 35:29

    Lac Operon Model

  • 38:38

    Viruses Introduction

  • 40:43

    Lytic and Lysogenic Cycles

  • 43:51

    HIV and Reverse Transcriptase

  • 45:25

    Transformation Lab Intro

  • 47:35

    Lab Procedure Details

  • 50:10

    pGLO Plasmid Explanation

  • 51:41

    Lab Results Interpretation

  • 53:45

    AP Test Relevance

  • 55:18

    Lab Guide and Analysis

  • 56:52

    Final Questions and Wrap-Up

  • 59:26

    Stream Conclusion

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Study Content & Tools

Study GuidesFRQs + AI ScoringAP Essay Grader for StudentsAI Grading for TeachersPractice QuestionsGlossaryScore Calculators

Company

What's New?PricingBulk DiscountsTestimonialsFAQsEmail us

Resources

AP Exam Schedule 2027AP Exam AccommodationsAP ClassesAP ClassroomEasiest AP ClassesHardest AP ClassesAP vs IBAP vs Dual EnrollmentAP vs Honors ClassesAP Scholar Awards

history

🌎 ap world history🇺🇸 ap us history🇪🇺 ap european history

social science

✊🏿 ap african american studies🗳️ ap comparative government🚜 ap human geography💶 ap macroeconomics🤑 ap microeconomics🧠 ap psychology👩🏾‍⚖️ ap us government

english & capstone

✍🏽 ap english language📚 ap english literature🔍 ap research💬 ap seminar

arts

🎨 ap art & design🖼️ ap art history🎵 ap music theory

science

🧬 ap biology🧪 ap chemistry♻️ ap environmental science🎡 ap physics 1🧲 ap physics 2💡 ap physics c: e&m⚙️ ap physics c: mechanics

math & computer science

🧮 ap calculus ab♾️ ap calculus bc📐 ap precalculus📊 ap statistics💻 ap computer science a⌨️ ap computer science p

world languages

🇨🇳 ap chinese🇫🇷 ap french🇩🇪 ap german🇮🇹 ap italian🇯🇵 ap japanese🏛️ ap latin🇪🇸 ap spanish language💃🏽 ap spanish literature

career kickstart

💼 ap business with personal finance🔒 ap cybersecurity

go beyond AP

high school exams

✏️ PSAT🎓 Digital SAT🎒 ACT

honors classes

🍬 honors algebra II🫀 honors anatomy🐇 honors biology👩🏽‍🔬 honors chemistry💲 honors economics📐 honors geometry⚾️ honors physics📏 honors pre-calculus📊 honors statistics🗳️ honors us government🇺🇸 honors us history🌎 honors world history

college classes

👩🏽‍🎤 arts👔 business🎤 communications🏗️ engineering📓 humanities➗ math🧑🏽‍🔬 science💶 social science

hs classes

🏛️ alabama history⛰️ appalachian studies🌴 california history🌍 earth science🌐 ethnic studies🐊 florida history🍑 georgia history🌎 global studies🌺 hawaiian studies📰 journalism🪶 native american studies🌵 new mexico history🤠 oklahoma history⚗️ physical science🎙️ speech and debate🤝 texas government🤠 texas history🌲 washington state history🕊️ world religions
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© 2026 Fiveable Inc. All rights reserved.

AP® and SAT® are trademarks registered by the College Board, which is not affiliated with, and does not endorse this website.