AP Biology Unit 8 practice questions
Try 10 multiple-choice questions from Unit 8 here. Pick an answer to see why it's right or wrong, then take the timed test above for a full AP-style set.
Question 1
Nutrient runoff from a farm causes an algal bloom in a nearby pond. The density of algal cells increases from 1,200 cells/mL on Day 1 to 96,000 cells/mL on Day 5. What is the average rate of change in algal cell density per day during this period?
19,200 cells/mL per day
23,700 cells/mL per day
24,000 cells/mL per day
94,800 cells/mL per day
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Answer: (B)
Calculating the rate of change requires dividing the change in population (94,800 cells/mL) by the time interval (4 days).
Question 2
A researcher measures mercury concentrations in a marine food web affected by industrial pollution. Phytoplankton contain 0.05 ppm of mercury, while sharks at the top of the food chain contain 32.0 ppm. How many times greater is the mercury concentration in the sharks compared to the phytoplankton?
6.4 times greater
64 times greater
640 times greater
6,400 times greater
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Answer: (C)
Calculating the ratio requires dividing the concentration in the top predator (32.0 ppm) by the concentration in the producer (0.05 ppm).
Question 3
Deforestation for agriculture reduced a tropical forest fragment from 800 hectares in 1990 to 320 hectares in 2020. What is the percent change in the area of the forest fragment over this 30-year period?
A decrease of 40%
A decrease of 60%
A decrease of 150%
A decrease of 250%
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Answer: (B)
Calculating percent change requires finding the difference (-480) and dividing by the initial value (800), then converting to a percentage.
Question 4
Atmospheric data indicates that CO2 levels increased from 350 ppm in 1980 to 410 ppm in 2020 due to human activities. What is the average annual rate of increase in CO2 concentration during this period?
1.5 ppm per year
8.8 ppm per year
10.3 ppm per year
60.0 ppm per year
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Answer: (A)
Calculating the rate requires dividing the total change in concentration (60 ppm) by the time interval (40 years).
Question 5
A population of 900 deer lives in an ecosystem with a carrying capacity (K) of 1000. If the maximum per capita growth rate (r_max) is 1.0, calculate the population growth rate (dN/dt) considering density-dependent negative feedback.
90 individuals/year, reflecting resource limits
100 individuals/year, reflecting resource limits
900 individuals/year, reflecting exponential growth
1000 individuals/year, reflecting exponential growth
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Answer: (A)
Calculates logistic growth correctly: 1.0 * 900 * ((1000-900)/1000) = 90.
Question 6
A student places 100 isopods in a choice chamber with wet and dry sides to test humidity preference. After 10 minutes, 70 isopods are on the wet side and 30 are on the dry side. Assuming no preference (null hypothesis), the expected count is 50 per side. The critical value for with 1 degree of freedom is 3.84. What is the chi-square value and conclusion?
8.0; fail to reject the null hypothesis
16.0; reject the null hypothesis
16.0; fail to reject the null hypothesis
32.0; reject the null hypothesis
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Answer: (B)
Calculating the chi-square statistic involves summing the squared differences divided by expected values for both groups: . Since 16.0 exceeds the critical value of 3.84, the null hypothesis is rejected.
Question 7
In a phototropism experiment, 200 seedlings are exposed to unidirectional light. Observed results show 140 bending toward light and 60 growing straight. Under the null hypothesis of random growth, expected counts are 100 each. The critical value at is 3.84. Which choice correctly describes the statistical outcome?
Chi-square is 16.0; light significantly affects growth direction
Chi-square is 32.0; light significantly affects growth direction
Chi-square is 32.0; light has no significant effect on growth
Chi-square is 64.0; light has no significant effect on growth
Show answer
Answer: (B)
The chi-square calculation is . Because 32.0 is greater than 3.84, the null hypothesis is rejected, indicating a significant response to light.
Question 8
Researchers test phototaxis in 60 fruit flies, observing 40 move to light and 20 move to darkness. If the null hypothesis states no preference, the expected count is 30 per group. With 1 degree of freedom, the critical value is 3.84. What is the result of the chi-square test?
3.33; fail to reject the null hypothesis
6.67; fail to reject the null hypothesis
6.67; reject the null hypothesis
13.33; reject the null hypothesis
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Answer: (C)
The calculation is . Since 6.67 > 3.84, the null hypothesis is rejected, showing a significant preference.
Question 9
Ninety fish are released into a tank with equal areas of sand, gravel, and mud. Observed counts are 50 on sand, 30 on gravel, and 10 on mud. The null hypothesis predicts 30 fish per area. The critical value for 2 degrees of freedom at is 5.99. What is the chi-square value and biological interpretation?
13.33; fish show no significant substrate preference
26.67; fish show a significant substrate preference
26.67; fish show no significant substrate preference
40.00; fish show a significant substrate preference
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Answer: (B)
The calculation sums contributions from all three groups: Sand () + Gravel () + Mud () = 26.67. This exceeds 5.99, indicating a significant preference.
Question 10
Fifty caterpillars are offered corn or tomato leaves. Thirty choose corn and 20 choose tomato. The null hypothesis predicts equal preference (25 each). The critical value at is 3.84. Which statement correctly reports the chi-square value and conclusion?
1.0; fail to reject the null hypothesis
2.0; fail to reject the null hypothesis
2.0; reject the null hypothesis
4.0; reject the null hypothesis
Show answer
Answer: (B)
The calculation is . Since 2.0 is less than 3.84, the null hypothesis cannot be rejected, meaning no significant preference is shown.
What's on the AP Biology Unit 8 practice test
The standard version takes about 60 minutes and follows the timing and directions of the AP exam, sized to one unit:
- 20 Multiple Choice Questions (30 minutes)
- 1 FRQ – FRQ 1 – Interpreting and Evaluating Experimental Results (Long) (15 minutes)
- 1 FRQ – FRQ 3 – Scientific Investigation (Short) (15 minutes)
Questions are drawn from 3469 multiple-choice questions and 101 FRQs written for Unit 8, Ecology.
Practice AP Biology Unit 8 FRQs
Answer the unit's free-response questions one at a time and get a score on every part.
- FRQ 1 – Interpreting and Evaluating Experimental Results (Long) (16 questions)
- FRQ 2 – Interpreting and Evaluating Experimental Results with Graphing (Long) (13 questions)
- FRQ 3 – Scientific Investigation (Short) (21 questions)
- FRQ 4 – Conceptual Analysis (Short) (16 questions)
- FRQ 5 – Analyze Model or Visual Representation (Short) (19 questions)
- FRQ 6 – Analyze Data (Short) (16 questions)
Review Unit 8 before you start
- 8.1 Responses to the Environment
- 8.2 Energy Flow Through Ecosystems
- 8.3 Population Ecology
- 8.4 Effect of Density of Populations
- 8.5 Community Ecology
- 8.6 Biodiversity
- 8.7 Disruptions to Ecosystems