Intercalating agent

Definition

An intercalating agent is a molecule that inserts itself between the base pairs of DNA, causing structural distortion. This can lead to mutations during DNA replication.

5 Must Know Facts For Your Next Test

1. Intercalating agents cause frameshift mutations by inserting between DNA base pairs.
2. Common examples include ethidium bromide, acridine orange, and proflavin.
3. Frameshift mutations induced by intercalating agents can result in nonfunctional proteins.
4. These agents are often used in laboratories for staining nucleic acids because they fluoresce under UV light.
5. Their mutagenic properties make them useful tools in genetic research but also potential carcinogens.

Review Questions

Related terms

Mutagen:

An agent that causes genetic mutation.

Frameshift Mutation:

A genetic mutation caused by insertions or deletions of a number of nucleotides not divisible by three.

Carcinogen:

A substance capable of causing cancer in living tissue.