Pairwise-end sequencing

Definition

Pairwise-end sequencing is a method used in genomics to sequence both ends of a DNA fragment. This technique helps in obtaining more accurate and comprehensive information about the genome structure.

5 Must Know Facts For Your Next Test

1. Pairwise-end sequencing involves sequencing the forward and reverse ends of DNA fragments.
2. It is often used to improve the accuracy of genome assemblies.
3. The method helps in identifying structural variations such as insertions, deletions, and inversions.
4. Pairwise-end sequencing provides longer reads that can span repetitive regions in the genome.
5. It is commonly employed in whole-genome sequencing projects for better mapping and annotation.

Related terms

Whole-Genome Sequencing:

A process that involves determining the complete DNA sequence of an organism's genome at a single time.

Structural Variations: Large-scale differences in genomic DNA sequences, such as insertions, deletions, duplications, and inversions.

Reads: Short sequences generated from DNA fragments during sequencing processes; these sequences are later assembled into a full genome.