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🦠microbiology review

Multiple cloning site (MCS)

Written by the Fiveable Content Team • Last updated September 2025
Written by the Fiveable Content Team • Last updated September 2025

Definition

A multiple cloning site (MCS) is a short segment of DNA containing multiple unique restriction sites. It is used in genetic engineering to facilitate the insertion of foreign DNA into a plasmid vector.

5 Must Know Facts For Your Next Test

  1. An MCS is also known as a polylinker.
  2. The presence of multiple restriction sites allows for flexibility in choosing enzymes for cloning.
  3. MCSs are typically found within plasmid vectors used for gene cloning.
  4. Insertion into an MCS can disrupt reporter genes, allowing for selection of recombinant clones.
  5. MCSs enable researchers to insert DNA fragments without altering the function of the vector.

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