🦠microbiology review

High-efficiency particulate air (HEPA) filter

Written by the Fiveable Content Team • Last updated August 2025
Written by the Fiveable Content Team • Last updated August 2025

Definition

A high-efficiency particulate air (HEPA) filter is a type of air filter that can remove at least 99.97% of airborne particles as small as 0.3 micrometers. HEPA filters are crucial for maintaining sterile environments by trapping microorganisms and other contaminants.

Course connection

Topic 13.2: 13.2 Using Physical Methods to Control Microorganisms

Unit 13

5 Must Know Facts For Your Next Test

  1. HEPA filters are used in various settings, including laboratories, hospitals, and cleanrooms, to control microbial growth.
  2. These filters work through a combination of interception, impaction, and diffusion mechanisms to capture particles.
  3. HEPA filters do not kill microorganisms but physically trap them within the filter media.
  4. To maintain effectiveness, HEPA filters must be regularly inspected and replaced according to manufacturer guidelines.
  5. The performance standards for HEPA filters are defined by organizations such as the Institute of Environmental Sciences and Technology (IEST).