IMAC, or Immobilized Metal Affinity Chromatography, is a technique used for the purification of proteins and peptides that possess a specific affinity for metal ions. This method utilizes metal ions, such as nickel or cobalt, which are covalently attached to a solid support to capture proteins that contain polyhistidine tags or other metal-binding motifs. IMAC is a crucial tool in the protein purification process, enabling researchers to isolate target proteins from complex mixtures based on their unique binding properties.
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