rt-qpcr, or reverse transcription quantitative polymerase chain reaction, is a laboratory technique used to measure the amount of specific RNA in a sample by first converting it into complementary DNA (cDNA) through reverse transcription and then amplifying that cDNA to quantify the RNA levels. This method is particularly significant for studying gene expression and the roles of RNA in various biological processes, including post-transcriptional regulation through mechanisms such as RNA interference and microRNAs.
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