Shuttle vectors

Definition

Shuttle vectors are plasmids engineered to replicate in two different host organisms, typically a prokaryote and a eukaryote. They are essential tools for transferring genetic material between different biological systems.

5 Must Know Facts For Your Next Test

1. Shuttle vectors contain origins of replication for both prokaryotic and eukaryotic hosts.
2. They often include selectable markers for identifying successful transformations in both types of hosts.
3. Shuttle vectors facilitate the study of gene function across different species.
4. They can carry large DNA fragments, making them useful for cloning large genes or genomic libraries.
5. Shuttle vectors are pivotal in biotechnological applications such as the production of recombinant proteins.

Review Questions

• What is the primary purpose of a shuttle vector?
• Why do shuttle vectors need to have multiple origins of replication?
• How do selectable markers work in the context of shuttle vectors?

Related terms

Plasmid: A small, circular piece of DNA that replicates independently from the chromosomal DNA.

Selectable Marker: A gene introduced into a cell that confers a trait suitable for artificial selection.

Recombinant Protein: A protein that is produced from recombinant DNA technology, often using modified host cells.