🦠microbiology review

Sabouraud's dextrose agar

Written by the Fiveable Content Team • Last updated September 2025
Written by the Fiveable Content Team • Last updated September 2025

Definition

Sabouraud's dextrose agar is a type of fungal culture medium specifically designed to isolate and cultivate dermatophytes and other types of fungi. It contains peptones, dextrose, and a low pH to inhibit bacterial growth while promoting fungal growth.

5 Must Know Facts For Your Next Test

  1. Sabouraud's dextrose agar has a low pH (around 5.6) which helps suppress bacterial growth.
  2. This medium is particularly useful for isolating fungi from clinical specimens, including those causing respiratory infections.
  3. It commonly contains antibiotics like chloramphenicol or gentamicin to further prevent bacterial contamination.
  4. Sabouraud's dextrose agar can be modified with cycloheximide to inhibit the growth of saprophytic fungi, allowing for better isolation of pathogenic species.
  5. Fungi such as Aspergillus and Candida are frequently isolated using this medium.

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