Fluorescence microscope

Definition

A fluorescence microscope is a type of optical microscope that uses fluorescence to generate an image. It relies on the emission of light by fluorophores to visualize structures within biological specimens.

5 Must Know Facts For Your Next Test

1. Fluorescence microscopes use specific wavelengths of light to excite fluorophores, which then emit light at different wavelengths.
2. They are essential in microbiology for visualizing cellular components, pathogens, and immune responses.
3. Common applications include EIAs (enzyme immunoassays) and ELISAs (enzyme-linked immunosorbent assays) where fluorescent labels are used.
4. Fluorescent antibody techniques leverage these microscopes to detect the presence of specific antigens or antibodies.
5. The main parts include a light source, filters, dichroic mirrors, and objective lenses designed for high sensitivity and specificity.

Related terms

Fluorophore: A molecule that emits light upon excitation by specific wavelengths.

Dichroic Mirror: An optical component that reflects certain wavelengths while transmitting others.

ELISA:

A plate-based assay technique for detecting and quantifying substances such as proteins, hormones, and antibodies.