ELISA

Definition

ELISA (Enzyme-Linked Immunosorbent Assay) is a technique used to detect the presence of antibodies or antigens in a sample. It is widely used in microbiology for diagnostics and research.

5 Must Know Facts For Your Next Test

1. ELISA can be used to detect both bacterial and viral pathogens through specific antibodies or antigens.
2. The four main types of ELISA are direct, indirect, sandwich, and competitive assays.
3. Color change in an ELISA test indicates the presence of the target antigen or antibody, quantified by measuring absorbance.
4. ELISA is pivotal in diagnosing infections like HIV, hepatitis, and various bacterial skin infections.
5. Proper sample preparation and controls are critical for accurate ELISA results.

Review Questions

Related terms

Antigen:

A molecule capable of inducing an immune response.

Antibody:

A protein produced by the immune system that binds specifically to a particular antigen.

Pathogen:

An organism that causes disease.