BSL-3

Definition

BSL-3 (Biosafety Level 3) is a containment protocol required for work involving microbes that can cause serious and potentially lethal diseases via inhalation. It includes strict laboratory practices, safety equipment, and facility design to prevent infection.

5 Must Know Facts For Your Next Test

1. BSL-3 laboratories require specialized ventilation systems to ensure air flows from clean areas to potentially contaminated areas.
2. Personnel must wear appropriate personal protective equipment (PPE), such as gloves, gowns, and respiratory protection.
3. All procedures in BSL-3 labs are performed within biological safety cabinets or other physical containment devices.
4. Access to BSL-3 laboratories is restricted and controlled at all times to protect laboratory workers and the environment.
5. Examples of microorganisms handled in BSL-3 labs include Mycobacterium tuberculosis, SARS-CoV-2, and Bacillus anthracis.

Review Questions

• What types of microbes necessitate the use of a BSL-3 laboratory?
• Name three key safety features employed in a BSL-3 lab.
• Why is it crucial to have specialized ventilation systems in BSL-3 laboratories?

Related terms

BSL-1:

The lowest biosafety level, dealing with well-characterized agents not known to cause disease in healthy humans.

BSL-2:

Involves agents that pose moderate hazards and requires limited access with enhanced precautionary measures.

BSL-4:

The highest biosafety level for dangerous and exotic agents posing high individual risk of aerosol-transmitted infections.