Single Isomorphous Replacement (SIR) is a method used in crystallography for determining the three-dimensional structure of a crystal by utilizing a known isomorphous compound as a reference. This technique involves replacing a single atom in the crystal with a heavier atom, allowing differences in scattering to be analyzed. The resulting differences in intensity between the native and derivative data are used to derive phase information, which is crucial for constructing an electron density map of the structure.
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