Southern blotting

Definition

Southern blotting is a laboratory technique used to detect specific DNA sequences within a complex mixture. It involves transferring DNA from a gel onto a membrane, followed by hybridization with a labeled probe.

5 Must Know Facts For Your Next Test

1. Southern blotting was named after its inventor, Edwin Southern.
2. The procedure includes steps such as DNA digestion, gel electrophoresis, transfer to a membrane, and probe hybridization.
3. This technique is crucial for identifying gene mutations and genetic fingerprinting.
4. Radioactive or fluorescent probes are commonly used for detecting specific DNA sequences.
5. Southern blotting can distinguish between different alleles of a gene based on DNA fragment sizes.

Review Questions

• What are the main steps involved in the Southern blotting process?
• Why is the use of labeled probes important in Southern blotting?
• How does Southern blotting contribute to the field of genetics?

Related terms

Northern Blotting: A technique similar to Southern blotting but used for detecting RNA instead of DNA

Western Blotting: A method used for detecting specific proteins in a sample through antibody binding

DNA Electrophoresis: A process that separates DNA fragments by size using an electric field