Written by the Fiveable Content Team • Last updated September 2025
Written by the Fiveable Content Team • Last updated September 2025
Definition
Southern blotting is a laboratory technique used to detect specific DNA sequences within a complex mixture. It involves transferring DNA from a gel onto a membrane, followed by hybridization with a labeled probe.
5 Must Know Facts For Your Next Test
Southern blotting was named after its inventor, Edwin Southern.
The procedure includes steps such as DNA digestion, gel electrophoresis, transfer to a membrane, and probe hybridization.
This technique is crucial for identifying gene mutations and genetic fingerprinting.
Radioactive or fluorescent probes are commonly used for detecting specific DNA sequences.
Southern blotting can distinguish between different alleles of a gene based on DNA fragment sizes.
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Related terms
Northern Blotting: A technique similar to Southern blotting but used for detecting RNA instead of DNA
Western Blotting: A method used for detecting specific proteins in a sample through antibody binding
DNA Electrophoresis: A process that separates DNA fragments by size using an electric field