Written by the Fiveable Content Team • Last updated September 2025
Written by the Fiveable Content Team • Last updated September 2025
Definition
Radiation hybrid mapping is a technique used to determine the order and distance between genes on chromosomes. It involves fragmenting DNA using radiation and then analyzing which fragments contain specific markers or genes.
5 Must Know Facts For Your Next Test
Radiation hybrid mapping uses ionizing radiation to break DNA into fragments.
The method relies on integrating these fragments into a host cell line, typically rodent cells.
Markers are identified by determining which DNA fragments were retained in the host cells after integration.
It is highly useful for constructing high-resolution maps of human and other genomes.
This technique can map both coding and non-coding regions of the genome.
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Related terms
Genetic Mapping: A process used to determine the location of genes on a chromosome.
Physical Mapping: A method that determines the physical distance between genetic markers on a chromosome.
Linkage Analysis: A genetic analysis technique that looks for co-segregation of traits within families to identify gene locations.