Genomic libraries

Definition

Genomic libraries are collections of DNA fragments that represent the entire genome of an organism. These libraries are used in genetic research for sequencing, mapping, and analyzing genes.

5 Must Know Facts For Your Next Test

1. Genomic libraries are created by fragmenting the DNA of an organism and cloning these fragments into vectors.
2. They are essential tools for identifying and isolating specific genes from a complex genome.
3. The process often involves the use of bacterial artificial chromosomes (BACs) or yeast artificial chromosomes (YACs) as vectors.
4. Screening genomic libraries can be done using techniques like hybridization with labeled probes or PCR amplification.
5. Genomic libraries differ from cDNA libraries, which contain only expressed gene sequences without introns.

Review Questions

Related terms

Vector: A vehicle used to transfer genetic material to a target cell, often plasmids, BACs, or YACs in genomic studies

cDNA Library: A collection of complementary DNA sequences derived from mRNA transcripts, representing only expressed genes

Hybridization:

A technique used to identify specific DNA sequences by allowing single-stranded probes to form double-stranded hybrids with target DNA