Biophysical Chemistry

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Turnover Number

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Biophysical Chemistry

Definition

Turnover number (often denoted as k\_cat) is a measure of the efficiency of an enzyme, defined as the number of substrate molecules converted to product by an enzyme molecule per unit time when the enzyme is fully saturated with substrate. This concept connects to reaction rates by illustrating how quickly a reaction can occur under optimal conditions and highlights the role of enzymes in speeding up reactions, offering insights into mechanisms of biochemical processes.

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5 Must Know Facts For Your Next Test

  1. Turnover number is typically expressed in units of reciprocal time, such as s\^{-1}, indicating how many substrate molecules an enzyme converts per second.
  2. A high turnover number reflects a highly efficient enzyme, while a low turnover number may indicate either a less efficient enzyme or one that operates under specific conditions.
  3. Turnover number is crucial in determining how enzymes function in metabolic pathways and how they can be targeted for inhibition in drug design.
  4. It is essential to consider turnover number in conjunction with substrate concentration and enzyme concentration to fully understand enzyme kinetics.
  5. The measurement of turnover number can vary based on experimental conditions such as pH, temperature, and the presence of inhibitors or activators.

Review Questions

  • How does turnover number reflect an enzyme's efficiency and its role in reaction rates?
    • Turnover number is a key indicator of an enzyme's efficiency because it quantifies how many substrate molecules are converted to product per unit time. A higher turnover number means that the enzyme can process substrates more quickly, directly impacting the overall reaction rate. Understanding this relationship allows for insights into how enzymes catalyze reactions and their potential applications in various biochemical processes.
  • Discuss how changes in substrate concentration can affect the turnover number and overall enzyme kinetics.
    • While turnover number itself is a property of an enzyme when it is fully saturated with substrate, changes in substrate concentration can significantly affect the apparent rate of reaction. At low substrate concentrations, the reaction rate increases linearly with concentration, but as saturation occurs, additional substrate has less impact due to limited active sites. This relationship is described by Michaelis-Menten kinetics, where at high concentrations, the reaction rate approaches maximum velocity (V\_max), influenced by turnover number.
  • Evaluate the significance of measuring turnover number in drug design and enzyme regulation strategies.
    • Measuring turnover number is critical for understanding how enzymes function within biological systems and can significantly influence drug design strategies. By knowing the turnover number, researchers can identify highly efficient enzymes that might be targeted for inhibition or modification. Additionally, drugs designed to influence enzyme activity must account for this measure to effectively modulate metabolic pathways, providing a pathway for developing more effective therapeutic interventions.
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