Fluorescence Resonance Energy Transfer (FRET) is a powerful technique used to measure the distance between two chromophores, typically in the range of 1-10 nanometers. It occurs when a donor fluorophore, upon excitation, transfers energy to an acceptor fluorophore without the emission of a photon. This non-radiative transfer is highly dependent on the distance and orientation of the two molecules, making it useful for studying molecular interactions and dynamics in biological systems.
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