Written by the Fiveable Content Team • Last updated September 2025
Verified for the 2026 exam
Verified for the 2026 exam•Written by the Fiveable Content Team • Last updated September 2025
Definition
Restriction enzymes are proteins that cut DNA at specific sequences, acting like molecular scissors. They are used in genetic engineering to cut and paste DNA fragments.
Related terms
Endonuclease: An enzyme that cuts DNA strands internally at specific sites, just like how restriction enzymes work.
Recognition Sequence: This is the specific sequence of nucleotides where a restriction enzyme will cut. It's like the dotted line on your craft paper telling you where to cut.
DNA Ligase: This is another type of enzyme that acts as glue, joining together two pieces of DNA after they've been cut by restriction enzymes.