Topoisomerase: An enzyme that helps relieve strain in the double helix ahead of the replication fork during replication by causing breaks and then rejoining them.
Primase: An enzyme that creates RNA primers on single-stranded sections of DNA during replication, providing starting points for synthesis by polymerases.
Single-Strand Binding Proteins (SSBPs): These proteins bind to separated single-strands during replication preventing them from re-forming a double helix before they can be used as templates.