🥼organic chemistry review

Sanger dideoxy method

Written by the Fiveable Content Team • Last updated August 2025
Written by the Fiveable Content Team • Last updated August 2025

Definition

The Sanger dideoxy method is a technique used for DNA sequencing by selectively incorporating chain-terminating dideoxynucleotides during DNA synthesis. It relies on the use of normal nucleotides and modified versions that prevent further extension, allowing the determination of the DNA sequence.

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