Ultramicrotome

Definition

An ultramicrotome is a precision instrument used to cut extremely thin sections of specimens for electron microscopy. It allows scientists to prepare samples with thicknesses on the order of nanometers.

5 Must Know Facts For Your Next Test

1. Ultramicrotomes are essential for preparing samples for transmission electron microscopy (TEM).
2. They can produce slices as thin as 50-70 nanometers, which is crucial for observing fine cellular structures.
3. The knife used in an ultramicrotome is often made from glass or diamond to achieve the necessary sharpness and precision.
4. Samples must be embedded in a hard resin before being sectioned by an ultramicrotome.
5. Properly prepared ultrathin sections can reveal detailed internal cellular architecture when stained and viewed under an electron microscope.

Related terms

Transmission Electron Microscopy (TEM): A technique that uses electrons to visualize thin specimens at very high resolutions.

Staining:

The process of adding dyes or other chemicals to specimens to enhance contrast under a microscope.

Embedding: The process of encasing a specimen in a supportive medium, like resin, before sectioning for microscopic examination.