upgrade
upgrade

Key Reproductive Hormones

Study smarter with Fiveable

Get study guides, practice questions, and cheatsheets for all your subjects. Join 500,000+ students with a 96% pass rate.

Get Started

Why This Matters

Reproductive hormones aren't just about making babies—they're your window into understanding feedback loops, endocrine signaling hierarchies, and target tissue specificity. In Bio 20300, you'll be tested on how these hormones communicate across the hypothalamic-pituitary-gonadal (HPG) axis, how negative feedback maintains homeostasis, and why timing matters (think pulsatile release patterns and cyclical changes). These concepts show up repeatedly in exam questions about endocrine regulation.

Don't just memorize which gland secretes what. Know why GnRH must be pulsatile, how the same hormone can have different effects in males versus females, and what happens when feedback mechanisms fail. If you understand the underlying logic—command hormones from the brain, tropic hormones from the pituitary, and effector hormones from the gonads—you'll nail both multiple choice and short-answer questions.


Hypothalamic and Pituitary Command Hormones

These hormones sit at the top of the reproductive hierarchy, acting as master regulators that control downstream hormone release. The hypothalamus integrates neural and hormonal signals, while the pituitary translates those signals into commands for the gonads.

Gonadotropin-Releasing Hormone (GnRH)

  • Secreted by the hypothalamus—this is the master switch for the entire reproductive axis
  • Pulsatile release pattern is critical; continuous GnRH actually suppresses FSH/LH (this is how some contraceptives work)
  • Stimulates anterior pituitary to release both FSH and LH, making it the upstream controller of fertility

Follicle-Stimulating Hormone (FSH)

  • Produced by anterior pituitary gonadotrophs—targets the gonads directly
  • In females: promotes ovarian follicle growth and estrogen production; in males: stimulates Sertoli cells to support spermatogenesis
  • Regulated by inhibin through negative feedback, giving the gonads a way to fine-tune their own stimulation

Luteinizing Hormone (LH)

  • Triggers ovulation in females when it surges mid-cycle—this is the event pregnancy tests indirectly detect
  • In males: stimulates Leydig cells to produce testosterone, making it essential for male sex hormone production
  • Works synergistically with FSH—neither hormone alone can sustain full reproductive function

Compare: FSH vs. LH—both are anterior pituitary gonadotropins regulated by GnRH, but FSH primarily supports gamete development (follicles/sperm) while LH drives hormone production and ovulation. Exam tip: if asked about the LH surge, connect it to positive feedback from estrogen.


Gonadal Steroid Hormones

These are the effector hormones—produced by the gonads, they carry out the actual work of developing sex characteristics, preparing for reproduction, and maintaining pregnancy. All are derived from cholesterol and are lipid-soluble, meaning they cross cell membranes and bind intracellular receptors.

Estrogen

  • Primary source is ovarian follicles (also placenta during pregnancy and adipose tissue in smaller amounts)
  • Drives female secondary sex characteristics—breast development, fat distribution, and bone maturation
  • Regulates the menstrual cycle by thickening the endometrium during the proliferative phase and triggering the LH surge via positive feedback

Progesterone

  • Secreted by the corpus luteum after ovulation (and later by the placenta during pregnancy)
  • Prepares and maintains the endometrium for implantation—converts it from proliferative to secretory phase
  • Inhibits uterine contractions and suppresses further ovulation, which is why it's called the "pro-gestation" hormone

Testosterone

  • Produced by Leydig cells in the testes (small amounts from ovaries and adrenal cortex in females)
  • Essential for spermatogenesis, male secondary sex characteristics, and maintaining libido in both sexes
  • Anabolic effects on muscle and bone explain why it's regulated as a controlled substance

Compare: Estrogen vs. Progesterone—both are ovarian steroids, but estrogen dominates the follicular phase (building the endometrium) while progesterone dominates the luteal phase (maintaining it). On an FRQ about the menstrual cycle, distinguish their timing and target tissue effects.


Feedback Regulators

These hormones fine-tune the system by providing negative feedback to the pituitary, preventing overproduction of gonadotropins. Without these brakes, the reproductive axis would spiral out of control.

Inhibin

  • Secreted by gonads—Sertoli cells in males, granulosa cells in females
  • Selectively inhibits FSH (not LH) at the anterior pituitary, providing precise control over gamete production
  • Creates a local feedback loop allowing the gonads to regulate their own stimulation independent of steroid hormones

Compare: Inhibin vs. Testosterone/Estrogen feedback—all provide negative feedback to the pituitary, but inhibin specifically targets FSH while gonadal steroids suppress both FSH and LH (and GnRH at the hypothalamus). Know which feedback pathway controls what.


Pregnancy and Lactation Hormones

These hormones support reproduction beyond conception—maintaining pregnancy, preparing for birth, and enabling nursing. They demonstrate how the endocrine system adapts to dramatically different physiological states.

Human Chorionic Gonadotropin (hCG)

  • Produced by the syncytiotrophoblast of the placenta beginning at implantation
  • Rescues the corpus luteum from degeneration, maintaining progesterone production until the placenta takes over (~10 weeks)
  • Detected in urine/blood pregnancy tests—doubles approximately every 48 hours in early pregnancy, making it a reliable marker

Prolactin

  • Anterior pituitary hormone that stimulates mammary gland development and milk synthesis
  • Normally inhibited by dopamine from the hypothalamus; suckling reduces this inhibition
  • Suppresses GnRH release, which is why breastfeeding can delay return of fertility (lactational amenorrhea)

Oxytocin

  • Synthesized in hypothalamus, released from posterior pituitary—note this is neurosecretion, not typical endocrine release
  • Stimulates uterine smooth muscle contractions during labor via positive feedback (Ferguson reflex)
  • Triggers milk ejection reflex (let-down) and plays roles in bonding and social behavior

Compare: Prolactin vs. Oxytocin in lactation—prolactin makes the milk (synthesis), oxytocin releases it (ejection). Both increase with suckling, but through different mechanisms. This distinction is a common exam question.


Quick Reference Table

ConceptBest Examples
HPG axis command hormonesGnRH, FSH, LH
Gonadal steroidsEstrogen, Progesterone, Testosterone
Negative feedback to pituitaryInhibin, Testosterone, Estrogen, Progesterone
Pregnancy maintenancehCG, Progesterone
Lactation hormonesProlactin (synthesis), Oxytocin (ejection)
Positive feedback examplesEstrogen → LH surge; Oxytocin → uterine contractions
Anterior pituitary hormonesFSH, LH, Prolactin
Posterior pituitary hormonesOxytocin

Self-Check Questions

  1. Which two hormones are both produced by the anterior pituitary and both regulated by GnRH, yet have distinct target cells in the gonads? What does each target?

  2. Compare the roles of estrogen and progesterone in the menstrual cycle—during which phase does each dominate, and what is each preparing the endometrium to do?

  3. How does inhibin's feedback mechanism differ from testosterone's feedback mechanism in males? Which pituitary hormone does each primarily suppress?

  4. If the corpus luteum degenerates before the placenta can produce sufficient progesterone, what would happen to the pregnancy? Which hormone normally prevents this, and how?

  5. Contrast prolactin and oxytocin in breastfeeding: which hormone is responsible for milk production versus milk release, and what stimulus increases both?