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🛡️Immunobiology

Key Functions of Antigen Presenting Cells

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Why This Matters

Antigen presenting cells (APCs) are the critical link between your innate and adaptive immune systems—and understanding how they function is essential for exam success. You're being tested on concepts like MHC-mediated antigen presentation, T cell activation requirements, and the coordination between humoral and cell-mediated immunity. APCs don't just passively display antigens; they actively shape whether your immune system mounts an attack, tolerates a substance, or forms long-lasting memory.

When you study APCs, focus on where each cell type operates, which MHC class it uses, and which lymphocytes it activates. These details determine whether you're looking at a cytotoxic response, a helper response, or antibody production. Don't just memorize cell names—know what immunological principle each APC demonstrates and how they work together to create a coordinated defense.


Professional Antigen Presenters: The Dendritic Cell Family

These cells are the professional APCs—their primary job is capturing antigens in peripheral tissues and delivering them to lymphoid organs where T cell activation occurs. They express high levels of MHC molecules and co-stimulatory signals, making them uniquely efficient at priming naïve T cells.

Dendritic Cells

  • Primary activators of naïve T cells—they're the only APCs capable of initiating primary adaptive immune responses in lymph nodes
  • Cross-presentation capability allows them to load exogenous antigens onto MHC class I molecules, enabling CD8+ cytotoxic T cell responses against viruses and tumors
  • Migration from tissues to lymph nodes is triggered by pathogen encounter, carrying processed antigens to where naïve T cells reside

Langerhans Cells

  • Skin-resident dendritic cells that form the immunological first line of defense at epithelial barriers
  • Express Langerin (CD207)—a unique C-type lectin receptor that distinguishes them from other dendritic cell subsets
  • Balance immunity and tolerance by determining whether skin antigens trigger responses or are tolerated, crucial for preventing allergic reactions

Compare: Dendritic cells vs. Langerhans cells—both are professional APCs that migrate to lymph nodes and activate naïve T cells, but Langerhans cells are tissue-specific (skin/mucosa) while conventional dendritic cells are distributed throughout most tissues. If an FRQ asks about cutaneous immunity or barrier defense, Langerhans cells are your go-to example.


Dual-Function APCs: Innate Meets Adaptive

These cells serve as APCs while also performing other critical immune functions. Their antigen presentation typically occurs in the context of their primary roles—phagocytosis for macrophages, antibody production for B cells.

Macrophages

  • Phagocytic APCs that engulf pathogens and present processed antigens via MHC class II to CD4+ T helper cells
  • Cytokine production polarizes T cell responses—secreting IL-12 drives Th1 differentiation, while IL-10 promotes regulatory responses
  • Functional plasticity allows them to shift between pro-inflammatory (M1) and tissue-repair (M2) phenotypes depending on microenvironment signals

B Cells

  • Antigen-specific APCs that use their B cell receptor (BCR) to capture and internalize specific antigens for presentation to T helper cells
  • Cognate T-B interaction via MHC class II presentation is required for B cell activation, germinal center entry, and class switching
  • Dual output—activated B cells differentiate into antibody-secreting plasma cells while also generating memory B cells for long-term protection

Compare: Macrophages vs. B cells as APCs—macrophages are nonspecific phagocytes that present whatever they engulf, while B cells selectively capture antigens matching their BCR. This means B cells present antigens at much lower concentrations but only their specific antigen, making them essential for T-dependent antibody responses.


Specialized B Cell Support: Follicular Dendritic Cells

These cells occupy a unique niche in the immune system—they support B cell responses but function completely differently from other APCs.

Follicular Dendritic Cells

  • Do NOT express MHC class II—unlike all other APCs, they don't present processed peptides to T cells
  • Display intact antigens on their surface using complement receptors (CR1, CR2) and Fc receptors, allowing B cells to sample native antigen during affinity maturation
  • Essential for germinal center reactions—they provide the antigen depots that drive B cell selection, ensuring only high-affinity B cells survive and differentiate

Compare: Conventional dendritic cells vs. follicular dendritic cells—despite similar names, they serve completely different functions. Conventional DCs activate T cells via MHC-peptide complexes, while FDCs support B cell maturation by displaying unprocessed antigens. Don't confuse them on exams—FDCs are stromal cells, not bone marrow-derived APCs.


Quick Reference Table

ConceptBest Examples
Naïve T cell activationDendritic cells, Langerhans cells
MHC class II presentationDendritic cells, Macrophages, B cells
Cross-presentation (MHC class I)Dendritic cells
T-dependent B cell activationB cells (as APCs to T helper cells)
Germinal center supportFollicular dendritic cells
Barrier/tissue immunityLangerhans cells, Macrophages
Cytokine-mediated T cell polarizationMacrophages, Dendritic cells
Antigen-specific captureB cells (via BCR)

Self-Check Questions

  1. Which two APC types are capable of activating naïve T cells, and what shared features make this possible?

  2. A patient has defective cross-presentation. Which APC function is impaired, and what type of immune response would be compromised?

  3. Compare and contrast how macrophages and B cells capture antigens for presentation—what does this difference mean for the specificity of their APC function?

  4. Why are follicular dendritic cells considered distinct from other APCs, even though they display antigens to lymphocytes?

  5. An FRQ asks you to trace the pathway from skin infection to T cell activation. Which APCs are involved, and what is the sequence of events?