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🦠Cell Biology

Cell Organelles Functions

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Why This Matters

Cell organelles aren't just a vocabulary list—they're the foundation for understanding how life works at the molecular level. When you study organelles, you're really learning about compartmentalization, the principle that cells divide labor among specialized structures to maximize efficiency. This concept connects directly to membrane transport, protein synthesis pathways, energy transformation, and cellular communication—all heavily tested topics on your exam.

Here's the key insight: exam questions rarely ask you to simply name an organelle. Instead, you're being tested on how organelles work together in pathways, why certain organelles share evolutionary origins, and what happens when organelle function breaks down. Don't just memorize what each organelle does—know what concept each one illustrates and how it connects to the bigger picture of cellular function.


Information Storage and Gene Expression

The cell's ability to store, protect, and express genetic information depends on specialized structures that keep DNA organized and accessible. Gene expression is tightly regulated through compartmentalization—separating transcription in the nucleus from translation in the cytoplasm.

Nucleus

  • Command center of the cell—houses DNA organized into chromosomes and controls all cellular activities through gene regulation
  • Nuclear envelope (double membrane with pores) separates genetic material from cytoplasm, allowing selective transport of mRNA and proteins
  • Regulates the cell cycle by controlling DNA replication and coordinating gene expression in response to cellular signals

Ribosomes

  • Site of translation—converts mRNA sequences into polypeptide chains using the genetic code
  • Two locations matter: free ribosomes make cytoplasmic proteins; bound ribosomes on rough ER make secreted and membrane proteins
  • Composed of rRNA and proteins—their universal presence in all cells makes them targets for antibiotics that selectively inhibit bacterial ribosomes

Compare: Nucleus vs. Ribosomes—both essential for protein production, but the nucleus handles transcription (DNA → mRNA) while ribosomes handle translation (mRNA → protein). If an FRQ asks about gene expression, trace the pathway through both structures.


Energy Transformation Organelles

Cells need to convert energy from one form to another—either capturing it from sunlight or extracting it from food molecules. Both mitochondria and chloroplasts use electron transport chains embedded in internal membranes to generate ATP.

Mitochondria

  • Powerhouse of the cell—performs cellular respiration, converting glucose and O2O_2 into ATP (up to 36-38 ATP per glucose molecule)
  • Double membrane structure with folded inner membrane (cristae) that increases surface area for the electron transport chain
  • Contains its own circular DNA and ribosomes—evidence for the endosymbiotic theory, suggesting mitochondria were once free-living bacteria

Chloroplasts

  • Site of photosynthesis—captures light energy using chlorophyll and converts CO2CO_2 and H2OH_2O into glucose and O2O_2
  • Thylakoid membranes contain photosystems for light reactions; stroma is the site of the Calvin cycle (carbon fixation)
  • Also has its own DNA and ribosomes—like mitochondria, supports endosymbiotic origin from ancient cyanobacteria

Compare: Mitochondria vs. Chloroplasts—both have double membranes, their own DNA, and use chemiosmosis to make ATP. The key difference: chloroplasts capture energy from light, while mitochondria release energy from organic molecules. Expect questions linking both to endosymbiotic theory.


The Endomembrane System: Protein Processing Pathway

Proteins destined for secretion, membranes, or lysosomes travel through a connected network of organelles. This pathway demonstrates how compartmentalization allows sequential modification and quality control of proteins.

Endoplasmic Reticulum

  • Rough ER (studded with ribosomes) synthesizes proteins that enter the secretory pathway—these get folded and quality-checked here
  • Smooth ER synthesizes lipids, detoxifies drugs and poisons, and stores calcium ions in muscle cells
  • Continuous with the nuclear envelope—newly made proteins are packaged into transport vesicles heading to the Golgi

Golgi Apparatus

  • Molecular post office—receives proteins from the ER, modifies them, sorts them, and ships them to correct destinations
  • Glycosylation (adding carbohydrate tags) occurs here, creating glycoproteins essential for cell recognition and signaling
  • Cis face receives, trans face ships—vesicles bud off containing finished products destined for lysosomes, membranes, or secretion

Lysosomes

  • Digestive compartments—contain hydrolytic enzymes that break down macromolecules, pathogens, and damaged organelles
  • Maintain acidic pH (around 5) optimal for enzyme function; the membrane protects the rest of the cell from self-digestion
  • Essential for autophagy—recycling cellular components during starvation or removing defective organelles

Compare: Rough ER vs. Golgi Apparatus—both modify proteins, but ER handles initial folding and quality control while Golgi adds final modifications and sorts for delivery. Trace a secreted protein's path: ribosome → rough ER → Golgi → vesicle → plasma membrane.


Structural Support and Boundaries

Cells need physical organization—barriers that control what enters and exits, scaffolding that maintains shape, and storage compartments. These structures demonstrate how form follows function at the cellular level.

Cell Membrane

  • Fluid mosaic modelphospholipid bilayer with embedded proteins creates a selectively permeable barrier
  • Regulates transport through channels, carriers, and pumps; maintains concentration gradients essential for cell function
  • Receptor proteins enable cell signaling and communication—how cells respond to hormones, neurotransmitters, and growth factors

Cytoskeleton

  • Dynamic protein networkmicrofilaments (actin), intermediate filaments, and microtubules (tubulin) provide structure and enable movement
  • Tracks for intracellular transport—motor proteins walk along microtubules carrying vesicles and organelles to specific locations
  • Essential for cell division—spindle fibers (microtubules) separate chromosomes; contractile ring (microfilaments) pinches cell in two

Vacuoles

  • Large central vacuole in plant cells stores water and maintains turgor pressure—the force that keeps plants rigid
  • Storage compartment for nutrients, pigments, and toxic waste products the cell needs to isolate
  • Functions like lysosomes in some cells—can contain digestive enzymes and participate in breaking down cellular debris

Compare: Cell Membrane vs. Vacuole Membrane (Tonoplast)—both are phospholipid bilayers with selective permeability, but the plasma membrane controls what enters/exits the cell while the tonoplast controls what enters/exits the vacuole. Plant cells use both to regulate water balance.


Quick Reference Table

ConceptBest Examples
Energy transformationMitochondria, Chloroplasts
Endosymbiotic theory evidenceMitochondria, Chloroplasts (own DNA, double membrane, ribosomes)
Protein synthesis pathwayRibosomes → Rough ER → Golgi Apparatus
Genetic information flowNucleus (transcription), Ribosomes (translation)
Membrane-bound digestionLysosomes, Vacuoles
Structural supportCytoskeleton, Cell Membrane, Cell Wall (plants)
Lipid synthesisSmooth ER
Cell signalingCell Membrane (receptors), Nucleus (gene regulation)

Self-Check Questions

  1. Which two organelles provide evidence for endosymbiotic theory, and what three features do they share that support this hypothesis?

  2. Trace the pathway of a secreted protein from synthesis to export—which organelles does it pass through, and what happens at each step?

  3. Compare and contrast the functions of rough ER and smooth ER. Why might liver cells have extensive smooth ER while pancreatic cells have extensive rough ER?

  4. How do lysosomes and vacuoles demonstrate similar functions but serve different primary roles in animal versus plant cells?

  5. If a cell's Golgi apparatus stopped functioning, which cellular processes would be disrupted? Explain how this would affect protein targeting and lysosome formation.