Glutamate Dehydrogenase

Definition

Glutamate dehydrogenase is an enzyme that catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. It plays a crucial role in the catabolism of proteins by facilitating the breakdown of amino acids and the entry of their carbon skeletons into central metabolic pathways.

5 Must Know Facts For Your Next Test

1. Glutamate dehydrogenase is found in the mitochondria of cells and is present in a wide variety of organisms, including bacteria, plants, and animals.
2. The enzyme catalyzes the reversible reaction: Glutamate + NAD(P)+ ⇌ Alpha-Ketoglutarate + NH3 + NAD(P)H.
3. The reaction catalyzed by glutamate dehydrogenase is an important step in the catabolism of amino acids, particularly the amino acid glutamate.
4. The enzyme plays a key role in the regulation of nitrogen metabolism by controlling the balance between amino acid catabolism and biosynthesis.
5. Glutamate dehydrogenase activity is regulated by various factors, including allosteric effectors, post-translational modifications, and the availability of substrates and cofactors.

Review Questions

• Explain the role of glutamate dehydrogenase in the catabolism of proteins.
  • Glutamate dehydrogenase is a crucial enzyme in the catabolism of proteins. It catalyzes the deamination of the amino acid glutamate, converting it to alpha-ketoglutarate and ammonia. This reaction is an important step in the breakdown of amino acids, as it allows the carbon skeleton of glutamate to enter the citric acid cycle for energy production. By facilitating the entry of amino acid-derived carbon compounds into central metabolic pathways, glutamate dehydrogenase plays a key role in the overall catabolism of proteins.
• Describe how the activity of glutamate dehydrogenase is regulated and its significance in the context of nitrogen metabolism.
  • The activity of glutamate dehydrogenase is regulated by various factors, including allosteric effectors, post-translational modifications, and the availability of substrates and cofactors. This regulation is important in the context of nitrogen metabolism, as the enzyme controls the balance between amino acid catabolism and biosynthesis. When the enzyme is active, it facilitates the breakdown of glutamate, releasing ammonia. This ammonia can then be used for the synthesis of other nitrogen-containing compounds or excreted. By regulating the activity of glutamate dehydrogenase, the cell can fine-tune its nitrogen metabolism to meet its specific needs, ensuring a balance between amino acid catabolism and anabolic processes.
• Evaluate the significance of the reversible reaction catalyzed by glutamate dehydrogenase and its implications for cellular energy production and nitrogen homeostasis.
  • The reversible reaction catalyzed by glutamate dehydrogenase is significant because it allows the enzyme to play a dual role in cellular metabolism. When the reaction proceeds in the direction of deamination, glutamate is converted to alpha-ketoglutarate, which can then enter the citric acid cycle for energy production. This pathway is crucial for the catabolism of amino acids and the utilization of their carbon skeletons for energy generation. Conversely, the reverse reaction, which produces glutamate from alpha-ketoglutarate and ammonia, is important for nitrogen homeostasis. The ability to synthesize glutamate from ammonia allows the cell to incorporate nitrogen into new amino acids and other nitrogen-containing compounds. This reversibility of the glutamate dehydrogenase-catalyzed reaction enables the enzyme to serve as a critical link between amino acid catabolism, energy production, and nitrogen metabolism, underscoring its importance in cellular physiology.

Related terms

Deamination: The process of removing the amino group (NH2) from an amino acid, converting it to an alpha-keto acid.

Alpha-Ketoglutarate: An intermediate in the citric acid cycle, which is a key pathway for the oxidation of carbon compounds to generate energy.

Amino Acid Catabolism: The breakdown of amino acids to produce energy or to provide precursors for other metabolic processes.