Warthin-Starry silver-precipitate procedure

Definition

The Warthin-Starry silver-precipitate procedure is a histological staining method used to detect certain bacteria in tissue samples, particularly those responsible for respiratory infections. It utilizes silver nitrate to stain the organisms, making them visible under a microscope.

5 Must Know Facts For Your Next Test

1. The Warthin-Starry stain is particularly useful in identifying Helicobacter pylori and Bartonella henselae.
2. It is a silver-based staining technique that highlights bacteria as black or dark brown against a pale yellow background.
3. This procedure is often employed when other staining methods fail to clearly identify the presence of certain bacteria.
4. The Warthin-Starry stain can be used on both tissue biopsies and cytological preparations.
5. It requires careful handling and precise timing during the staining process to avoid artifacts.

Review Questions

• What types of bacteria are commonly identified using the Warthin-Starry silver-precipitate procedure?
• Why might the Warthin-Starry stain be preferred over other staining methods for certain bacterial infections?
• What are some key visual characteristics of bacteria stained with the Warthin-Starry method?

Related terms

Gram Stain: A common bacterial staining technique that differentiates bacteria into Gram-positive and Gram-negative based on cell wall composition.

Acid-Fast Stain: A differential stain used primarily to identify Mycobacterium species by their resistance to decolorization by acids.

Histology: The study of the microscopic structure of tissues.