Use-dilution test

Definition

A use-dilution test is a procedure used to evaluate the effectiveness of disinfectants and antiseptics by assessing their ability to prevent microbial growth on surfaces. This test involves exposing standardized surfaces with dried microorganisms to different concentrations of the chemical agent.

5 Must Know Facts For Your Next Test

1. The use-dilution test is standardized by the American Society for Testing and Materials (ASTM).
2. It typically involves bacterial species like Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella choleraesuis.
3. The process includes immersing contaminated cylinders into the disinfectant solution for a specific time period.
4. Following exposure, the cylinders are transferred to a growth medium to observe any surviving bacteria.
5. Results are measured based on whether there is no growth, indicating effectiveness, or growth, indicating ineffectiveness.

Related terms

Minimum Inhibitory Concentration (MIC):

The lowest concentration of an antimicrobial agent that prevents visible growth of a microorganism.

Zone of Inhibition:

A clear area surrounding an antimicrobial agent on an agar plate where bacteria cannot grow.

Phenol Coefficient Test: A measure that compares the disinfecting power of a compound to that of phenol under standard conditions.