Sticky ends

Definition

Sticky ends are short, single-stranded overhangs generated by the staggered cutting of DNA by certain restriction enzymes. These ends can easily pair with complementary sequences, facilitating the ligation of DNA fragments.

5 Must Know Facts For Your Next Test

1. Sticky ends are produced when restriction enzymes cut DNA at specific palindromic sequences.
2. They are called 'sticky' because their unpaired bases can hydrogen bond with complementary sequences.
3. Sticky ends are crucial for recombinant DNA technology as they allow for the precise joining of DNA fragments.
4. Enzymes like EcoRI and HindIII are known for creating sticky ends.
5. The ability to produce sticky ends is used in cloning, gene splicing, and various genetic engineering applications.

Review Questions

• What is the primary function of sticky ends in genetic engineering?
• Which type of enzyme generates sticky ends and how do they facilitate cloning?
• Name two commonly used restriction enzymes that create sticky ends.

Related terms

Blunt Ends: Short double-stranded DNA fragments without overhangs created by restriction enzymes.

Restriction Enzyme: Proteins that cut DNA at specific recognition sites, often producing sticky or blunt ends.

DNA Ligase: An enzyme that joins DNA fragments together by forming phosphodiester bonds between nucleotides.