Southwestern blot

Definition

Southwestern blot is a laboratory technique used to detect DNA-binding proteins by combining features of both Southern and Western blotting methods. It involves electrophoretic separation of proteins, followed by their transfer onto a membrane and probing with labeled DNA fragments.

5 Must Know Facts For Your Next Test

1. Southwestern blotting detects DNA-binding proteins rather than nucleic acids or antibodies.
2. The technique combines aspects of Southern blot (DNA detection) and Western blot (protein detection).
3. Proteins are first separated using SDS-PAGE and then transferred to a nitrocellulose or PVDF membrane.
4. A labeled DNA probe is used to identify specific DNA-binding proteins on the membrane.
5. This method is particularly useful for studying transcription factors and other regulatory proteins.

Review Questions

• What types of molecules does Southwestern blot specifically detect?
• Which two blotting techniques are combined in Southwestern blotting?
• What type of labeled probe is used in Southwestern blot?

Related terms

Western Blot: A technique used to detect specific proteins in a sample using antibodies.

Southern Blot: A method for detecting specific sequences of DNA within a mixture using hybridization.

Electrophoresis: A laboratory technique that uses an electric field to separate molecules based on size and charge.