Single-stranded binding proteins

Definition

Single-stranded binding proteins (SSBs) are essential during DNA replication, stabilizing the unwound DNA strands. They prevent the single strands from re-annealing or forming secondary structures.

5 Must Know Facts For Your Next Test

1. SSBs bind to single-stranded DNA regions created during replication.
2. They play a crucial role in maintaining the integrity of the replication fork.
3. SSBs prevent secondary structures that can impede replication processes.
4. These proteins are necessary for the proper function of DNA polymerase on the template strand.
5. Mutations or deficiencies in SSBs can lead to genomic instability and replication errors.

Review Questions

• What is the primary function of single-stranded binding proteins during DNA replication?
• How do SSBs contribute to maintaining the integrity of the replication fork?
• What potential issues could arise if single-stranded binding proteins are not functioning properly?

Related terms

Helicase:

An enzyme that unwinds the double-stranded DNA helix ahead of the replication fork.

DNA Polymerase:

The enzyme responsible for synthesizing new strands of DNA complementary to the template strands.

Primer: A short nucleic acid sequence that provides a starting point for DNA synthesis.