Resolution

Definition

Resolution is the ability of a microscope to distinguish two adjacent points as distinct and separate. Higher resolution allows for better clarity and detail in the microscopic image.

5 Must Know Facts For Your Next Test

1. Resolution is determined by the wavelength of light used and the numerical aperture of the lens.
2. Shorter wavelengths of light provide higher resolution.
3. Electron microscopes have higher resolution compared to light microscopes because electrons have shorter wavelengths than visible light.
4. The limit of resolution for a typical light microscope is about 200 nanometers.
5. Using immersion oil between the objective lens and the specimen can increase resolution by reducing light refraction.

Review Questions

• What factors influence the resolution of a microscope?
• Why do electron microscopes offer higher resolution than light microscopes?
• How does immersion oil improve the resolution in microscopy?

Related terms

Numerical Aperture: A measure of a microscope lens's ability to gather light and resolve fine specimen detail.

Wavelength:

The distance between successive peaks of a wave, which affects resolution in microscopy.

Electron Microscope: A type of microscope that uses electrons instead of light to achieve higher magnification and resolution.