Reporter gene

Definition

A reporter gene is a gene that researchers attach to a regulatory sequence of another gene to study the expression of the target gene. It produces an easily measurable product, allowing scientists to monitor and quantify genetic activity.

5 Must Know Facts For Your Next Test

1. Reporter genes are used as markers to indicate if a gene has been taken up by or expressed in the host organism.
2. Common reporter genes include GFP (green fluorescent protein), luciferase, and β-galactosidase.
3. When fused with promoter sequences, reporter genes help in studying promoter activity under different conditions.
4. Reporter assays can be used for screening drugs that affect gene expression or for functional genomics studies.
5. The choice of reporter gene depends on factors like sensitivity, ease of detection, and compatibility with the host organism.

Review Questions

Related terms

Promoter:

A DNA sequence that initiates transcription of a particular gene.

GFP (Green Fluorescent Protein): A protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.

Luciferase: An enzyme used as a reporter that catalyzes reactions producing bioluminescence, commonly from fireflies.