Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify and detect DNA sequences. It allows for the rapid and specific identification of genetic material from various sources, including viruses.
5 Must Know Facts For Your Next Test
PCR requires the use of specific primers that flank the DNA region of interest.
The process involves repeated cycles of denaturation, annealing, and extension.
Taq polymerase is commonly used in PCR due to its heat resistance.
PCR can be used to diagnose viral infections by amplifying viral genetic material present in a sample.
Real-time PCR (qPCR) enables quantitative measurement of DNA and is often used in diagnostics.
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Related terms
Primer: A short sequence of nucleotides that provides a starting point for DNA synthesis.