Phospholipid-derived fatty acid analysis (PLFA)

Definition

Phospholipid-derived fatty acid analysis (PLFA) is a method used to profile microbial communities by analyzing the phospholipid fatty acids in cell membranes. This technique helps characterize the structure and function of prokaryotic cells.

5 Must Know Facts For Your Next Test

1. PLFA can differentiate between various types of microorganisms based on their unique fatty acid profiles.
2. This method is valuable for studying microbial ecology and environmental microbiology.
3. PLFA analysis involves extracting lipids from cell membranes, converting them to fatty acid methyl esters, and then analyzing them using gas chromatography.
4. Different microbial groups have characteristic PLFA profiles that can indicate changes in community composition or metabolic status.
5. PLFA does not require culturing microorganisms, making it useful for studying complex environmental samples.

Review Questions

Related terms

Gas Chromatography:

A technique used to separate and analyze compounds that can be vaporized without decomposition.

Microbial Ecology:

The study of microorganisms in their natural environments.

Fatty Acid Methyl Ester (FAME): A derivative of a fatty acid that is formed through esterification with methanol, commonly analyzed in PLFA studies.