PAGE

Definition

Polyacrylamide gel electrophoresis (PAGE) is a technique used to separate macromolecules like DNA, RNA, and proteins based on their size and charge. The gel matrix acts as a sieve that differentiates molecules during an electric field application.

5 Must Know Facts For Your Next Test

1. PAGE can be used for both nucleic acids and proteins but requires specific conditions for each type.
2. SDS-PAGE is a variant where proteins are denatured using sodium dodecyl sulfate (SDS) to ensure they are separated based on size alone.
3. In native PAGE, proteins maintain their secondary and tertiary structures, allowing assessment of their functional forms.
4. Visualization of the separated molecules often involves staining techniques such as Coomassie Blue or silver staining for proteins.
5. PAGE can be coupled with Western blotting to detect specific proteins using antigen-antibody complexes.

Review Questions

• What is the purpose of using SDS in SDS-PAGE?
• How does native PAGE differ from SDS-PAGE?
• What visualization methods are commonly used after performing PAGE?

Related terms

Agarose Gel Electrophoresis: A method similar to PAGE but typically used for separating larger DNA or RNA fragments.

Western Blotting: A technique that involves transferring proteins separated by PAGE onto a membrane followed by detection using antibodies.

Electrophoresis Buffer: A solution that conducts electricity and helps maintain pH during electrophoresis.