Northern blot

Definition

A northern blot is a laboratory technique used to detect specific RNA sequences in a sample by hybridizing them with complementary DNA probes. It allows researchers to study gene expression and RNA processing.

5 Must Know Facts For Your Next Test

1. Northern blotting involves the separation of RNA molecules by gel electrophoresis.
2. RNA samples are transferred from the gel to a membrane, usually made of nylon or nitrocellulose.
3. The RNA on the membrane is then hybridized with labeled DNA or RNA probes that are complementary to the target sequence.
4. Detection methods for the labeled probes can include radioactive isotopes, chemiluminescence, or fluorescence.
5. Northern blotting is particularly useful for studying the size, abundance, and integrity of specific mRNA molecules.

Related terms

Southern Blot: A technique used to detect specific DNA sequences by hybridizing them with complementary DNA probes.

Western Blot:

A method used to detect specific proteins in a sample using antibody binding.

Hybridization Probe: A fragment of DNA or RNA used to detect the presence of complementary sequences by binding through base pairing.