Next generation sequencing

Definition

Next Generation Sequencing (NGS) is a high-throughput method used to determine the sequence of nucleotides in DNA and RNA. It allows for rapid sequencing of large stretches of genetic material, providing detailed insights into microbial genetics.

5 Must Know Facts For Your Next Test

1. NGS can sequence an entire genome within a day, significantly faster than traditional methods.
2. It enables the study of microbial communities by identifying and quantifying various microorganisms present in a sample.
3. NGS can be used to detect genetic mutations, including those responsible for antibiotic resistance in bacteria.
4. The technology involves parallel sequencing, where millions of small fragments of DNA are sequenced simultaneously.
5. Applications of NGS include metagenomics, transcriptomics, and epigenetics in microbiological research.

Review Questions

• What are some key applications of Next Generation Sequencing in microbiology?
• How does Next Generation Sequencing improve our ability to study microbial communities?
• What advantage does NGS have over traditional sequencing methods in terms of speed and data output?

Related terms

Metagenomics:

The study of genetic material recovered directly from environmental samples.

Transcriptomics:

The study of RNA transcripts produced by the genome under specific circumstances or in a specific cell type.

Epigenetics:

The study of heritable changes in gene expression that do not involve changes to the underlying DNA sequence.