Microinjection

Definition

Microinjection is a technique used to introduce foreign DNA or other substances directly into a cell using a fine needle. It is commonly employed in genetic engineering to manipulate microbial genomes.

5 Must Know Facts For Your Next Test

1. Microinjection allows for precise insertion of genetic material at the single-cell level.
2. The technique requires specialized equipment such as a micromanipulator and an inverted microscope.
3. It can be used to create transgenic organisms by injecting DNA into embryos or zygotes.
4. Microinjection can be applied to a variety of cells, including bacterial, plant, and animal cells.
5. Despite its precision, microinjection is labor-intensive and requires high levels of skill.

Review Questions

• What are the main advantages of using microinjection in genetic engineering?
• Describe the equipment necessary for performing a microinjection procedure.
• How does microinjection differ from other methods of introducing foreign DNA into cells?

Related terms

Transformation: The process by which foreign DNA is introduced into a cell, causing it to acquire new genetic traits.

Transfection: A method for introducing nucleic acids into eukaryotic cells via non-viral means.

Electroporation: A technique that uses electrical pulses to create temporary pores in cell membranes through which DNA can enter.