Written by the Fiveable Content Team • Last updated September 2025
Written by the Fiveable Content Team • Last updated September 2025
Definition
An in-use test is a method used to evaluate the effectiveness of antiseptics and disinfectants under actual conditions of use. It measures the reduction in microbial load on surfaces or instruments after application of the disinfectant.
5 Must Know Facts For Your Next Test
In-use tests are performed in real-world settings, such as hospitals or laboratories, rather than controlled laboratory conditions.
These tests help determine if a disinfectant remains effective over time and under typical usage conditions.
In-use tests often involve sampling surfaces before and after disinfection to quantify microbial reduction.
Results can be influenced by factors such as the presence of organic matter, temperature, and humidity.
In-use testing is essential for validating cleaning protocols and ensuring compliance with health regulations.
Review Questions
Related terms
Disinfectant Efficacy Test: A laboratory-based test that determines how effectively a disinfectant kills microorganisms.
Microbial Load: The number of microorganisms present on a surface or object.