Hemocytometer

Definition

A hemocytometer is a specialized counting chamber used to count cells in a given volume of liquid. It is commonly used in microbiology to estimate the concentration of microbes in a sample.

5 Must Know Facts For Your Next Test

1. The hemocytometer consists of a thick glass microscope slide with a grid etched into it.
2. It allows for the precise counting of cells by providing defined areas and volumes for easier calculation.
3. To use the hemocytometer, a small volume of cell suspension is placed under a coverslip on the grid.
4. The number of cells within certain squares of the grid can be counted and then used to calculate the concentration per unit volume.
5. Proper dilution and mixing are crucial when preparing samples to ensure accurate counts.

Review Questions

• What is the primary purpose of using a hemocytometer in microbiology?
• How does the grid on a hemocytometer assist in cell counting?
• Why is proper sample preparation important when using a hemocytometer?

Related terms

Microscope: An instrument used to view objects that are too small to be seen by the naked eye.

Cell Count: The process of determining the number of cells in a specific volume or area.

Dilution: The process of reducing the concentration of solutes in solution, often used prior to counting cells.