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Gravity displacement autoclave

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Microbiology

Definition

A gravity displacement autoclave is a type of steam sterilizer that removes air through gravity, allowing steam to displace the air and effectively sterilize medical and laboratory equipment. It operates at high temperatures and pressures to kill all forms of microbial life, including spores.

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5 Must Know Facts For Your Next Test

  1. Gravity displacement autoclaves typically operate at temperatures between 121°C and 134°C.
  2. The process usually takes about 15-30 minutes depending on the load and temperature settings.
  3. They are commonly used in hospitals, laboratories, and other healthcare facilities for sterilizing instruments.
  4. Effective sterilization requires proper loading to ensure steam penetration to all surfaces.
  5. Biological indicators, like spore tests, are often used to validate the efficacy of the autoclave cycle.

Review Questions

  • What temperature ranges do gravity displacement autoclaves typically operate at?
  • Why is it important to properly load items in a gravity displacement autoclave?
  • How is the effectiveness of a gravity displacement autoclave cycle commonly validated?

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