🦠microbiology review

Geobacillus stearothermophilus

Written by the Fiveable Content Team • Last updated August 2025
Written by the Fiveable Content Team • Last updated August 2025

Definition

Geobacillus stearothermophilus is a thermophilic bacterium commonly used as a biological indicator for sterilization processes. It is highly resistant to heat and can survive at temperatures up to 75°C.

5 Must Know Facts For Your Next Test

  1. Geobacillus stearothermophilus spores are used to test the efficacy of autoclaves and other sterilization methods.
  2. It thrives in hot environments, making it an ideal candidate for studying heat resistance.
  3. This bacterium can form endospores, which are highly resistant to extreme conditions.
  4. In microbiology labs, it serves as a control organism to ensure that sterilization protocols are effective.
  5. The presence of Geobacillus stearothermophilus after a sterilization cycle indicates that the process was not effective.

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