Direct Coombs’ test

Definition

The direct Coombs’ test detects antibodies that are attached to the surface of red blood cells. It is used to diagnose conditions such as autoimmune hemolytic anemia.

5 Must Know Facts For Your Next Test

1. The direct Coombs' test is also known as the direct antiglobulin test (DAT).
2. It involves adding Coombs reagent, which contains anti-human globulin, to a patient's red blood cells.
3. A positive result indicates that antibodies or complement proteins are bound to the surface of red blood cells.
4. This test is crucial for diagnosing hemolytic diseases in newborns, such as hemolytic disease of the newborn (HDN).
5. It can also be used to investigate transfusion reactions and autoimmune disorders.

Review Questions

• What is the primary purpose of the direct Coombs' test?
• How does a positive direct Coombs' test result help in diagnosing hemolytic anemia?
• Why might the direct Coombs' test be important for newborns?

Related terms

Indirect Coombs' Test: Detects free-floating antibodies in the serum that could bind to red blood cells.

Hemolytic Disease of the Newborn (HDN): A condition where maternal antibodies attack fetal red blood cells.

Autoimmune Hemolytic Anemia: An autoimmune disorder where the immune system destroys its own red blood cells.