Blunt ends

Definition

Blunt ends are DNA fragments with no overhanging bases at either end. They are produced when DNA is cut straight across by certain restriction enzymes.

5 Must Know Facts For Your Next Test

1. Blunt ends can be created using restriction enzymes like SmaI and EcoRV.
2. They are less efficient for ligation compared to sticky ends due to the lack of complementary overhangs.
3. Blunt end ligation requires higher concentrations of DNA and ligase enzyme.
4. Blunt ends are often used in cloning vectors where precision is required for inserting DNA fragments.
5. They can be converted into sticky ends using linkers or adaptors for more efficient ligation.

Review Questions

• Which type of restriction enzyme typically creates blunt ends?
• Why is blunt end ligation less efficient compared to sticky end ligation?
• What methods can be used to convert blunt ends into sticky ends?

Related terms

Sticky Ends: DNA fragments with overhanging bases that can easily pair with complementary sequences.

Restriction Enzyme: Proteins that cut DNA at specific sequences, producing either blunt or sticky ends.

Ligation: The process of joining two DNA fragments together, typically facilitated by the enzyme DNA ligase.