Biological safety cabinet

Definition

A biological safety cabinet (BSC) is an enclosed, ventilated laboratory workspace designed to protect the user, experimental material, and the environment from biohazards and contamination. It uses high-efficiency particulate air (HEPA) filters to clean the air that flows in and out of the cabinet.

5 Must Know Facts For Your Next Test

1. There are three classes of BSCs: Class I, Class II, and Class III, each providing different levels of protection.
2. Class II BSCs are the most commonly used in clinical and research laboratories.
3. HEPA filters in BSCs can remove at least 99.97% of airborne particles sized 0.3 micrometers or larger.
4. Proper use of a BSC includes practices such as avoiding rapid movements and arranging materials logically to prevent contamination.
5. BSCs must be regularly certified by trained professionals to ensure they are functioning correctly.

Review Questions

• What are the primary functions of a biological safety cabinet?
• How does a HEPA filter contribute to the effectiveness of a BSC?
• What practices should be followed to maintain sterility when working within a BSC?

Related terms

HEPA Filter: High-Efficiency Particulate Air filter that removes at least 99.97% of airborne particles sized 0.3 micrometers or larger.

Laminar Flow Hood: A device similar to a BSC but primarily used for protecting samples from contamination rather than providing biohazard protection.

Autoclave: A device that uses steam under pressure to sterilize equipment and other items, effectively killing bacteria, viruses, fungi, and spores.